Viterbi Conference.
Talk by Michael Yaffee on Proteomic analysis of kinase signalling networks
- proteomic approach. how the information flows between the proteins.
- functionaly annotate proteome
- unify cell signaling and netw bilogy with bioinformatics
- identify druggable targets.
- - facilitate molecular analysis of chemical mechanisms
- provide a basis for understanding mutations.
- ctc2 kinase. Master regulator for cell division
- prevents the cells from dividing.
- we have a hierarchical process for phosphorylation and binding
domains.
- If we find motifs of the proteins .
Predict kinase substrates and binding domain.
pssm based on motifs from peptide libraryand phage display
experiments.
walk through the entire protein sequence db and score how good itis.
- Lets you make the call
- If the site were conserved.
Limitations:
1) Motifs based on peptides
2) pssms assume motif positions linearly independent.
- You can have pattern matchine
- HMM's or Neural networks
- don'tdo it as you need to have a large data set of mapped
phosphorlyations
- contribution from mass spectrometry
How does kinase signaling work.
If we wanted to assemble a very complicated machine.
- a concept that is fairly obvious. If you had a protein. Chk2engaged
in dna damage responses. After that is phosphorylated.
Talk by Michael Yaffee on Proteomic analysis of kinase signalling networks
- proteomic approach. how the information flows between the proteins.
- functionaly annotate proteome
- unify cell signaling and netw bilogy with bioinformatics
- identify druggable targets.
- - facilitate molecular analysis of chemical mechanisms
- provide a basis for understanding mutations.
- ctc2 kinase. Master regulator for cell division
- prevents the cells from dividing.
- we have a hierarchical process for phosphorylation and binding
domains.
- If we find motifs of the proteins .
Predict kinase substrates and binding domain.
pssm based on motifs from peptide libraryand phage display
experiments.
walk through the entire protein sequence db and score how good itis.
- Lets you make the call
- If the site were conserved.
Limitations:
1) Motifs based on peptides
2) pssms assume motif positions linearly independent.
- You can have pattern matchine
- HMM's or Neural networks
- don'tdo it as you need to have a large data set of mapped
phosphorlyations
- contribution from mass spectrometry
How does kinase signaling work.
If we wanted to assemble a very complicated machine.
- a concept that is fairly obvious. If you had a protein. Chk2engaged
in dna damage responses. After that is phosphorylated.
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